FLUORECARE FERRITIN (FERR)

The fluorecare® Ferritin, based on the principle of the
immunochromatographic assay, is used to detect the concentration of
Ferritin in human serum or plasma by double antibody sandwich method.
The human serum or plasma is added into the specimen adding hole, the
Ferritin in the specimens to be tested is combined with the
fluorescently-labeled Ferritin antibody on the conjugate pad, and the
Ferritin-antibody complex is formed. Because of the effect of the
chromatogaphy, Ferritin-antibody complex will be diffused along the
Nitrocellulose membrane. In the test line (T) region, Ferritin- antibody
complex will be combined with the coated antibody in the detection line area,
the higher concentration of Ferritin, the more aggregation of the complex on
the detection line, the deeper colour of the fluorescent band under the
specific wavelength. The signal intensity of fluorescent antibody is positively
correlated with the concentration of the substance to be measured in the
specimens.Fluorescently-labeled the mouse-anti-rabbit IgG antibody is
diffused to the quality control line (C) and is captured by the rabbit IgG
antibody to form a fluorescent band under the specific wavelength. When the
reaction is over, the instrument can be used to interpret the results. The
optical density of the control line and test line will be analyzed by the
instrument. Afterwards, the instrument calculates the concentration of the
Ferritin according to the formerly set calibration curve and displays the
results, with ng/mL as a unit.